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Beyotime
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Servicebio Inc
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Keygen Biotech
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Epizyme Inc
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Sangon Biotech
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Yeasen Biotechnology
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Brickell Biotech
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Journal: Bioactive Materials
Article Title: Synergistic targeting of senolytic and senomorphic action with dual-engineered biomimetic macrophage nanovesicles for mitigating osteoarthritis
doi: 10.1016/j.bioactmat.2025.11.047
Figure Lengend Snippet: Synthesis and characterization of BS@MD. (A) Representative TEM images of BS, BS@M, and BS@MD. Scale bar = 100 nm. (B) Hydrodynamic diameter and PDI, (C) Zeta potential of BS, BS@M, and BS@MD (n = 3). (D) Colloid stability of BS@MD in PBS and DMEM supplemented with 10 % FBS at 37 °C over 7 days (n = 3). (E) Fluorescence microscope images showing co-localization of the BS core (FITC, green) and macrophage membranes (Dil, red), with Pearson’s correlation coefficient of 0.75 ± 0.03, confirming successful core–shell assembly. Scale bar = 4 μm (left), 2 μm (middle), 500 nm (right). (F) SDS-PAGE analysis comparing protein profiles of RAW 264.7 lysate, membrane vesicles (MMs), and BS@M (equal protein loading). (G) Fluorescence microscope images of BS@M and BS@MD following staining with APC-labeled secondary antibody (APC-IgG), verifying successful conjugation of anti-DPP4 antibodies via DBCO–azide click chemistry. Scale bar = 50 μm. (H) In vitro release profiles of BTZ and Sab from BS and BS@MD in PBS at pH 5.0 and 7.4 over 24 h. Data are presented as mean ± SD. (I) Mechanism of pH-responsive cleavage of BS via breakage of catechol-boronate network.
Article Snippet: Hoechst 33342, DAPI solution, Lyso-Tracker Green, Cell Counting Kit-8 (CCK-8), Calcein-AM/PI Live/Dead cell double staining kit, membrane and cytosol protein extraction kit, BCA kit, and
Techniques: Zeta Potential Analyzer, Fluorescence, Microscopy, SDS Page, Membrane, Staining, Labeling, Conjugation Assay, In Vitro
Journal: Foods
Article Title: Unlocking the Functional Potential of Pecan Nut Cake: A Study on Bioactive Peptide Production
doi: 10.3390/foods15020323
Figure Lengend Snippet: ( A ) SDS-PAGE profile of PNC proteins. Lane M: molecular weight marker; Lanes 1–2: pecan peptides at a concentration of 0.05 g/mL; Lanes 3–5: pecan peptides at a concentration of 0.1 g/M ( A ); ( B – E ) effects of monoculture and co-culture fermentations with different bacterial strains on peptide and free amino acid contents in PNC. Different letters above the bars indicate significant differences ( p < 0.05) according to Duncan’s multiple range test; ( F – H ) antioxidant capacity and α-glucosidase inhibitory activity of bifunctional peptides from PNC.
Article Snippet: The
Techniques: SDS Page, Molecular Weight, Marker, Concentration Assay, Co-Culture Assay, Activity Assay